mRNA sequencing of Variovorax paradoxus or Bacillus mycoides exposed ... - SRA

SRX24669095: mRNA sequencing of Variovorax paradoxus or Bacillus mycoides exposed to plant or scrambled miRNA after 20 or 120 minutes
1 ILLUMINA (Illumina NovaSeq 6000) run: 42.1M spots, 8.5G bases, 2.5Gb downloads

Design: 1 mL of every culture was sampled and centrifuged at 5000 rpm for 5 min. The bacterial pellet was resuspended in 100 L of a lysozyme solution: 1 mg/mL for V. paradoxus (Gram -) and 3 mg/mL for B. mycoides (Gram +). After vortexing the resuspension, the samples were left to incubate for 15 min at room temperature. The following steps used solutions from the RNeasy Plant Mini Kit (QIAGEN). Total RNA was sent for sequencing at the Centre dexpertise et de services Gnome Qubec (Montreal, Canada), where ribosomal RNAs were depleted and messenger RNAs were sequenced.

Submitted by: Institut National de la Recherche Scientifique

Study: Effect of plant miRNAs on the transcriptome of Variovorax paradoxus and Bacillus mycoides

PRJNA1111827 • SRP509492 • All experiments • All runs

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We incubated Variovorax and Bacillus with a synthetic mixture of the six most abundant A. thaliana rhizosphere miRNAs: miR159a, miR159b, miR159c, miR161.1, miR158a and miR165b, or a mixture of scrambled miRNAs at the same concentration.

Sample: Bacillus mycoides, plant miRNA mix, 20min, rep1

SAMN41511804 • SRS21404143 • All experiments • All runs

Organism: Bacillus mycoides

Library:

Name: BM1_20min

Instrument: Illumina NovaSeq 6000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: Inverse rRNA

Layout: PAIRED

Runs: 1 run, 42.1M spots, 8.5G bases, 2.5Gb

Run	# of Spots	# of Bases	Size	Published
SRR29147755	42,054,543	8.5G	2.5Gb	2024-05-23

ID:: 32988069

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